Crystal structures of the active site in specifically metal-depleted and cobalt-substituted horse liver alcohol dehydrogenase derivatives.

@article{Schneider1983CrystalSO,
  title={Crystal structures of the active site in specifically metal-depleted and cobalt-substituted horse liver alcohol dehydrogenase derivatives.},
  author={G. R. Eugenia Schneider and Hans Eklund and Eila S. Cedergren-Zeppezauer and Michael Zeppezauer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1983},
  volume={80 17},
  pages={
          5289-93
        }
}
Two derivatives of horse liver alcohol dehydrogenase (LADH) in which the active site is specifically metal-depleted [H4Zn(n)2LADH] or specifically Co-substituted [Co(c)2-Zn(n)2LADH] have been studied by crystallographic methods. (In these formulae, "n" identifies the noncatalytic zinc ion and "c" identifies the catalytic metal ion.) X-ray data were collected for H4Zn(n)2LADH to 2.7-A resolution and for Co(c)2Zn(n)2LADH to 2.4-A resolution. Difference Fourier maps demonstrate clearly that the… CONTINUE READING