Crystal structures of an A-form duplex with single-adenosine bulges and a conformational basis for site-specific RNA self-cleavage.

@article{Portmann1996CrystalSO,
  title={Crystal structures of an A-form duplex with single-adenosine bulges and a conformational basis for site-specific RNA self-cleavage.},
  author={Stefan Portmann and Stefan Grimm and Christopher Workman and Nassim Usman and Martin Egli},
  journal={Chemistry & biology},
  year={1996},
  volume={3 3},
  pages={173-84}
}
BACKGROUND Bulged nucleotides are common secondary structural motifs in RNA molecules and are often involved in RNA-RNA and RNA-protein interactions. RNA is selectively cleaved at bulge sites (when compared to other sites within stems) in the presence of divalent metal cations. The effects of bulge nucleotides on duplex stability and topology have been extensively investigated, but no detailed X-ray structures of bulge-containing RNA fragments have been available. RESULTS We have crystallized… CONTINUE READING