Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment.

  title={Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment.},
  author={I Vidovi{\'c} and Stephanie Nottrott and Klaus Hartmuth and Reinhard L{\"u}hrmann and Ralf Ficner},
  journal={Molecular cell},
  volume={6 6},
We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from… CONTINUE READING
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