Crystal structure of the nucleosome core particle at 2.8 Å resolution
@article{Luger1997CrystalSO, title={Crystal structure of the nucleosome core particle at 2.8 {\AA} resolution}, author={Karolin Luger and Armin W. M{\"a}der and Robin K. Richmond and David F. Sargent and Timothy J. Richmond}, journal={Nature}, year={1997}, volume={389}, pages={251-260} }
The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it. Both histone/histone and histone/DNA interactions depend on the histone fold domains and additional, well ordered structure elements extending from this motif. Histone amino-terminal tails pass over and between the gyres of the DNA superhelix to contact neighbouring particles. The…
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References
SHOWING 1-10 OF 51 REFERENCES
Structure of the nucleosome core particle at 7 Å resolution
- ChemistryNature
- 1984
The crystal structure of the nucleosome core particle has been solved to 7 Å resolution and the central turn of superhelix and H3 · H4 tetramer have dyad symmetry, but the H2A · H2B dimers show departures due to interparticle associations.
X-ray diffraction analysis of crystals containing twofold symmetric nucleosome core particles.
- ChemistryActa crystallographica. Section D, Biological crystallography
- 1996
Nucleosome core particles containing a DNA palindrome and purified chicken erythrocyte histone octamer have been reconstituted and crystallized to ensure that the structure determined by X-ray diffraction will yield a true representation of the DNA strand rather than the twofold averaged structure which would result from using a non-palindromic DNA sequence.
Crystals of a nucleosome core particle containing defined sequence DNA.
- Biology, ChemistryJournal of molecular biology
- 1988
Characterization of nucleosome core particles containing histone proteins made in bacteria.
- BiologyJournal of molecular biology
- 1997
Nucleosome core particles containing native and mutant histones made in bacteria have facilitated its X-ray structure determination at 2.8 A resolution.
The effect of nucleosome phasing sequences and DNA topology on nucleosome spacing.
- BiologyJournal of molecular biology
- 1996
It is found that nucleosome repeats directed by a strong positioning sequence are dominated by the cation-induced spacing as well as by the effects of topology, which is described as a new parameter that influences nucleosomal spacing in a predictable way.
The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix.
- ChemistryProceedings of the National Academy of Sciences of the United States of America
- 1991
The structure of the octameric histone core of the nucleosome has been determined by x-ray crystallography to a resolution of 3.1 A and the folded histone chains are elongated rather than globular and are assembled in a characteristic "handshake" motif, which is named the histone fold.
Mapping nucleosome position at single base-pair resolution by using site-directed hydroxyl radicals.
- Biology, ChemistryProceedings of the National Academy of Sciences of the United States of America
- 1996
A base-pair resolution method for determining nucleosome position in vitro has been developed to com- plement existing, less accurate methods and is potentially adaptable for determine nucleosomes position in chromatin in vivo.
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin
- BiologyThe Journal of cell biology
- 1979
It is concluded that H1 stabilizes the nucleosome and is located in the region of the exit and entry points of the DNA in H1-depleted chromatin, which has the form of an unravelled filament.