Crystal structure of the archaeal asparagine synthetase: interrelation with aspartyl-tRNA and asparaginyl-tRNA synthetases.

@article{Blaise2011CrystalSO,
  title={Crystal structure of the archaeal asparagine synthetase: interrelation with aspartyl-tRNA and asparaginyl-tRNA synthetases.},
  author={Micka{\"e}l Blaise and Mathieu Fr{\'e}chin and Vincent Oli{\'e}ric and Christophe Charron and Claude Sauter and Bernard Lorber and Herv{\'e} Roy and Daniel M. Kern},
  journal={Journal of molecular biology},
  year={2011},
  volume={412 3},
  pages={437-52}
}
Asparagine synthetase A (AsnA) catalyzes asparagine synthesis using aspartate, ATP, and ammonia as substrates. Asparagine is formed in two steps: the β-carboxylate group of aspartate is first activated by ATP to form an aminoacyl-AMP before its amidation by a nucleophilic attack with an ammonium ion. Interestingly, this mechanism of amino acid activation… CONTINUE READING