Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å

@article{Umena2011CrystalSO,
  title={Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 {\AA}},
  author={Yasufumi Umena and Keisuke Kawakami and Jian-Ren Shen and Nobuo Kamiya},
  journal={Nature},
  year={2011},
  volume={473},
  pages={55-60}
}
Photosystem II is the site of photosynthetic water oxidation and contains 20 subunits with a total molecular mass of 350 kDa. The structure of photosystem II has been reported at resolutions from 3.8 to 2.9 Å. These resolutions have provided much information on the arrangement of protein subunits and cofactors but are insufficient to reveal the detailed structure of the catalytic centre of water splitting. Here we report the crystal structure of photosystem II at a resolution of 1.9 Å. From our… Expand
Structure of the catalytic, inorganic core of oxygen-evolving photosystem II at 1.9 Å resolution.
TLDR
The high resolution structure of the catalytic center provided a solid basis for elucidation of the mechanism of photosynthetic water splitting, and the structural features of the Mn(4)CaO(5)-cluster analyzed at 1.9 Å are reviewed. Expand
Structure of Sr-substituted photosystem II at 2.1 Å resolution and its implications in the mechanism of water oxidation
TLDR
An apparent elongation in the bond distance between Sr and one of the two terminal water ligands of Ca2+, W3, whereas that of the Sr-W4 distance was not much changed may contribute to the decrease of oxygen evolution upon Sr2+-substitution, and suggests a weak binding and rather mobile nature of this particular water molecule (W3). Expand
The Structure of Photosystem II and the Mechanism of Water Oxidation in Photosynthesis.
  • Jian-Ren Shen
  • Chemistry, Medicine
  • Annual review of plant biology
  • 2015
TLDR
The overall structure of PSII is provided followed by detailed descriptions of the specific structure of the Mn4CaO5 cluster and its surrounding protein environment, based on the geometric organization revealed by the crystallographic analysis. Expand
Oxygen‐evolving Photosystem II
Photosystem II is the enzyme that catalyzes the thermodynamically demanding splitting of water, yielding dioxygen, protons, and electrons, a sunlight-driven reaction that forms the foundation ofExpand
Photosystem II: the water-splitting enzyme of photosynthesis.
  • J. Barber
  • Chemistry, Biology
  • Cold Spring Harbor symposia on quantitative biology
  • 2012
TLDR
The overall structure of the catalytic site provides a framework to propose a mechanistic scheme for the water-splitting process and gives a blueprint for the development of catalysts that mimick the reaction in an artificial chemical system as a means to generate solar fuels. Expand
Heterogeneous Composition of Oxygen-Evolving Complexes in Crystal Structures of Dark-Adapted Photosystem II.
TLDR
It is found that the two OECs of dark-adapted PSII dimers in crystals are not fully synchronized in the S1 state, indicating heterogeneity in the OEC composition. Expand
Structure of photosystem II and substrate binding at room temperature
TLDR
Ammonia, a water analogue, has been used as a marker, as it binds to the Mn4CaO5 cluster in the S2 and S3 states, and this approach, together with a comparison of the native dark and 2F states, is used to discriminate between proposed O–O bond formation mechanisms. Expand
Electronic structure of the oxygen-evolving complex in photosystem II prior to O-O bond formation
TLDR
Frequency, multidimensional magnetic resonance spectroscopy reveals that all four manganese ions of the catalyst are structurally and electronically similar immediately before the final oxygen evolution step; they all exhibit a 4+ formal oxidation state and octahedral local geometry. Expand
Rationalizing the 1.9 Å crystal structure of photosystem II--A remarkable Jahn-Teller balancing act induced by a single proton transfer.
TLDR
XRD structures of PS II at progressively improved resolution reveal the positions of bridging O atoms within the Mn4Ca core of the WOC for the first time, and an atomic resolution structure at 1.9 is produced, the most resolved to date. Expand
A Hydrogen-Bond Stabilized Mechanism of Oxygen Evolution in Photosystem II: A Proposed Computational Experiment
The ability of plants to take in water and release oxygen into the atmosphere is crucial to the survival of life on Earth. During photosynthesis, water is oxidized to O2 (dioxygen) at the OxygenExpand
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