Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus.

@article{MartnezFleites2005CrystalSO,
  title={Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus.},
  author={Carlos Mart{\'i}nez-Fleites and Miguel Ort{\'i}z-Lombard{\'i}a and Tirso Pons and Nicolas Tarbouriech and Edward J. Taylor and Juan G. Arrieta and Lazaro E. Hernandez and Gideon J Davies},
  journal={The Biochemical journal},
  year={2005},
  volume={390 Pt 1},
  pages={
          19-27
        }
}
The endophytic Gram-negative bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10), which converts sucrose into fructooligosaccharides and levan. The enzyme is included in GH (glycoside hydrolase) family 68 of the sequence-based classification of glycosidases. The three-dimensional structure of LsdA has been determined by X-ray crystallography at a resolution of 2.5 A (1 A=0.1 nm). The structure was solved by molecular replacement… CONTINUE READING
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