Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus.
@article{MartnezFleites2005CrystalSO,
title={Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus.},
author={Carlos Mart{\'i}nez-Fleites and Miguel Ort{\'i}z-Lombard{\'i}a and Tirso Pons and Nicolas Tarbouriech and Edward J. Taylor and Juan G. Arrieta and Lazaro E. Hernandez and Gideon J Davies},
journal={The Biochemical journal},
year={2005},
volume={390 Pt 1},
pages={
19-27
}
}
The endophytic Gram-negative bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10), which converts sucrose into fructooligosaccharides and levan. The enzyme is included in GH (glycoside hydrolase) family 68 of the sequence-based classification of glycosidases. The three-dimensional structure of LsdA has been determined by X-ray crystallography at a resolution of 2.5 A (1 A=0.1 nm). The structure was solved by molecular replacement… CONTINUE READING