Crystal structure of an assembly intermediate of respiratory Complex II

@article{Sharma2017CrystalSO,
  title={Crystal structure of an assembly intermediate of respiratory Complex II},
  author={Pankaj Sharma and Elena Maklashina and Gary Cecchini and Tina M Iverson},
  journal={Nature Communications},
  year={2017},
  volume={9}
}
Flavin is covalently attached to the protein scaffold in ~10% of flavoenzymes. However, the mechanism of covalent modification is unclear, due in part to challenges in stabilizing assembly intermediates. Here, we capture the structure of an assembly intermediate of the Escherichiacoli Complex II (quinol:fumarate reductase (FrdABCD)). The structure contains the E. coli FrdA subunit bound to covalent FAD and crosslinked with its assembly factor, SdhE. The structure contains two global… 
13 Citations
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