Crystal structure of a new cyan fluorescent protein and its hue-shifted variants.

@article{Kikuchi2009CrystalSO,
  title={Crystal structure of a new cyan fluorescent protein and its hue-shifted variants.},
  author={Akihiro Kikuchi and Eiko Fukumura and Satoshi Karasawa and Yoshitsugu Shiro and Atsushi Miyawaki},
  journal={Biochemistry},
  year={2009},
  volume={48 23},
  pages={5276-83}
}
Green fluorescent protein (GFP) based techniques are well established in molecular biology; however, the detailed mechanism for the fine-tuning of fluorescent colors remains unclear. Here, we report the cloning and crystal structure of a new cyan-emitting GFP-like protein, KCy. We also developed a mutant protein with a high folding efficiency (KCy-G4219: lambda(abs) = 453 nm; lambda(em) = 486 nm). X-ray diffraction analysis revealed that the KCy chromophore is formed from an internal Ser62… CONTINUE READING

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