Crystal structure of a metal ion-bound IS200 transposase.

  title={Crystal structure of a metal ion-bound IS200 transposase.},
  author={Hyung Ho Lee and Ji Young Yoon and Hyoun Sook Kim and Ji Yong Kang and Kyoung Hoon Kim and Do Jin Kim and Jun Yong Ha and Bunzo Mikami and Hye Jin Yoon and Se Won Suh},
  journal={The Journal of biological chemistry},
  volume={281 7},
IS200 transposases, present in many bacteria and Archaea, appear to be distinct from other groups of transposases. To provide a structural basis for understanding the action of IS200 transposases, we have determined the crystal structure of the SSO1474 protein from Sulfolobus solfataricus, a member of the IS200 family, in both Mn(2+)-bound and Mn(2+)-free forms. Its monomer fold is distinct from other classes of structurally characterized transposases. Two monomers form a tight dimer by… CONTINUE READING