Crystal structure of a cytochrome P450 2B6 genetic variant in complex with the inhibitor 4-(4-chlorophenyl)imidazole at 2.0-A resolution.

@article{Gay2010CrystalSO,
  title={Crystal structure of a cytochrome P450 2B6 genetic variant in complex with the inhibitor 4-(4-chlorophenyl)imidazole at 2.0-A resolution.},
  author={Sean C Gay and Manish B Shah and Jyothi C. Talakad and Keiko Maekawa and Arthur G. Roberts and P Ross Wilderman and Ling Min Sun and Jane Y. X. Yang and Stephanie C. Huelga and Wen-xu Hong and Qinghai Zhang and Charles David Stout and James R. Halpert},
  journal={Molecular pharmacology},
  year={2010},
  volume={77 4},
  pages={
          529-38
        }
}
The structure of the K262R genetic variant of human cytochrome P450 2B6 in complex with the inhibitor 4-(4-chlorophenyl)imidazole (4-CPI) has been determined using X-ray crystallography to 2.0-A resolution. Production of diffraction quality crystals was enabled through a combination of protein engineering, chaperone coexpression, modifications to the purification protocol, and the use of unique facial amphiphiles during crystallization. The 2B6-4-CPI complex is virtually identical to the rabbit… CONTINUE READING

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