Crystal structure of a cyclic AMP-independent mutant of catabolite gene activator protein.

@article{Weber1987CrystalSO,
  title={Crystal structure of a cyclic AMP-independent mutant of catabolite gene activator protein.},
  author={Irene T. Weber and Gary L Gilliland and James Harman and Alan Peterkofsky},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 12},
  pages={5630-6}
}
Escherichia coli NCR91 synthesizes a mutant form of catabolite gene activator protein (CAP) in which alanine 144 is replaced by threonine. This mutant, which also lacks adenylate cyclase activity, has a CAP phenotype; in the absence of cAMP it is able to express genes that normally require cAMP. CAP91 has been purified and crystallized with cAMP under the same conditions as used to crystallize the wild type CAP X cAMP complex. X-ray diffraction data were measured to 2.4-A resolution and the… CONTINUE READING