Crystal structure of a [NiFe] hydrogenase maturation protease HybD from Thermococcus kodakarensis KOD1.

Abstract

A [NiFe] hydrogenase maturation protease HybD from Thermococcus kodakarensis KOD1 (TkHybD) is involved in the cleavage of the C-terminal residues of [NiFe] hydrogenase large subunits by Ni recognition. Here, we report the crystal structure of TkHybD at 1.82 Å resolution to better understand this process. TkHybD exhibits an α/β/α sandwich fold with conserved residues responsible for the Ni recognition. Comparisons of TkHybD with homologous proteins also reveal that they share a common overall architecture, suggesting that they have similar catalytic functions. Our results including metal binding site prediction provide insight into the substrate recognition and catalysis mechanism of TkHybD. Proteins 2016; 84:1321-1327. © 2016 Wiley Periodicals, Inc.

DOI: 10.1002/prot.25070

Cite this paper

@article{Kwon2016CrystalSO, title={Crystal structure of a [NiFe] hydrogenase maturation protease HybD from Thermococcus kodakarensis KOD1.}, author={Sunghark Kwon and Yuichi Nishitani and Satoshi Watanabe and Yoshinori Hirao and Tadayuki Imanaka and Tamotsu Kanai and Haruyuki Atomi and Kunio Miki}, journal={Proteins}, year={2016}, volume={84 9}, pages={1321-7} }