Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus.

Abstract

We have determined a 2.6A resolution crystal structure of Pfu DNA polymerase, the most commonly used high fidelity PCR enzyme, from Pyrococcus furiosus. Although the structures of Pfu and KOD1 are highly similar, the structure of Pfu elucidates the electron density of the interface between the exonuclease and thumb domains, which has not been previously… (More)
DOI: 10.1016/j.ijbiomac.2008.01.010

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