Crystal structure of CcdB, a topoisomerase poison from E. coli.

  title={Crystal structure of CcdB, a topoisomerase poison from E. coli.},
  author={Remy Loris and Minh-Hoa Dao-Thi and E M Bahassi and Laurence Van Melderen and Freddy Poortmans and Robert Liddington and Martine Couturier and Lode Wyns},
  journal={Journal of molecular biology},
  volume={285 4},
The crystal structure of CcdB, a protein that poisons Escherichia coli gyrase, was determined in three crystal forms. The protein consists of a five-stranded antiparallel beta-pleated sheet followed by a C-terminal alpha-helix. In one of the loops of the sheet, a second small three-stranded antiparallel beta-sheet is inserted that sticks out of the molecule as a wing. This wing contains the LysC proteolytic cleavage site that is protected by CcdA and, therefore, forms a likely CcdA recognition… CONTINUE READING