Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium.

@article{Wu2013CrystalSA,
  title={Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium.},
  author={Miao Wu and Gregg T Beckham and Anna M Larsson and Takuya Ishida and Seonah Kim and Christina M. Payne and Michael E. Himmel and Michael F. Crowley and Svein Jarle Horn and Bj\orge Westereng and Kiyohiko Igarashi and Masahiro Samejima and Jerry St{\aa}hlberg and Vincent G H Eijsink and Mats Sandgren},
  journal={The Journal of biological chemistry},
  year={2013},
  volume={288 18},
  pages={12828-39}
}
Carbohydrate structures are modified and degraded in the biosphere by a myriad of mostly hydrolytic enzymes. Recently, lytic polysaccharide mono-oxygenases (LPMOs) were discovered as a new class of enzymes for cleavage of recalcitrant polysaccharides that instead employ an oxidative mechanism. LPMOs employ copper as the catalytic metal and are dependent on oxygen and reducing agents for activity. LPMOs are found in many fungi and bacteria, but to date no basidiomycete LPMO has been structurally… CONTINUE READING

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Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium .
Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium .
A calculated potential energy surface for surface translation indicates that P. chrysosporium GH61D exhibits energy wells whose spacing seems adapted to the spacing of cellobiose units along a cellulose chain .
Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium .
Here we present the three - dimensional crystal structure of the basidiomycete Phanerochaete chrysosporium GH61D LPMO , and , for the first time , measure the product distribution of LPMO action on a lignocellulosic substrate .
Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium .
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