Crystal structure analysis of a complete turn of B-DNA

  title={Crystal structure analysis of a complete turn of B-DNA},
  author={Richard A. Wing and Horace R. Drew and Tsunehiro Takano and Chris Allen Broka and Shoji Tanaka and Keiichi Itakura and Richard E. Dickerson},
DNA is probably the most discussed and least observed of all biological macromolecules. Although its role in biology is a central one, with many examples such as operators and restriction sites where specific base sequences have control functions or interact with specific enzymes, the structures that DNA can adopt have been based until now only on sequence-averaged fibre diffraction patterns. Recent improvements in triester synthesis methods have made possible the preparation of sufficient… 
The anatomy of A-, B-, and Z-DNA.
Single-crystal x-ray analyses of double-stranded DNA molecules of predetermined sequence, with 4 to 12 base pairs, are carried out for understanding of the mode of bending of DNA in chromatin, and the way in which specific DNA sequences are recognized by drug molecules and repressor proteins.
Sequence dependence of the helical repeat of DNA in solution
The application of the band-shift method previously developed in this laboratory to the determination of the dependence of the DNA helical repeat in solution on its nucleotide sequence is reported.
New crystal structures of nucleic acids and their complexes.
One- and Two-Dimensional NMR Studies of the Structures of Simple Sequence DNAs
Progress towards understanding the biological functions of proteins and DNA at the molecular level is moving at a very rapid pace as a result of the development of DNA sequencing and footprinting
Structural variations inZ-DNA
In the laboratory, systematic crystallographic studies are made to explore the precise influence of sequence on the microstructure of Z-DNA, especially the effect of the introduction of A.T base pairs in sequences that otherwise consisted of C.G base pairs.
Structural variations in Z-DNA
The first crystal structure of Z-DNA, i.e. of the sequence d(CGCGCG)2 established many sequence-dependent micro- structural features. Nevertheless, a regular model of Z-DNA could be built for
Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H:DNA Complexes.
This work describes an alternative approach to determine crystal structures of the dodecamer in cases where molecular replacement does not produce a solution or when crystals of the DNA alone cannot be grown.
The helical periodicities of DNA in solution and in chromatin
Although X-ray analysis produces a wealth of information, it is also important to study the structure of DNA in solution, under physiological conditions, where the conformation of DNA is not affected by factors such as intermolecular interactions and localised high ionic conditions present in both fibres and crystals.


Helical repeat of DNA in solution.
  • J. Wang
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1979
The helical repeat of DNA in solution has been measured directly by analyzing the gel electrophoretic patterns of pairs of covalently closed DNAs with length differences between 1 and 58 base pairs,
Sequence-specific recognition of double helical nucleic acids by proteins.
It is concluded that a single hydrogen bond is inadequate for uniquely identifying any particular base pair, as this leads to numerous degeneracies, but using two hydrogen bonds, fidelity of base pair recognition may be achieved.
Molecular structure of a left-handed double helical DNA fragment at atomic resolution
The DNA fragment d(CpGpCpGpCpG) crystallises as a left-handed double helical molecule with Watson–Crick base pairs and an antiparallel organisation of the sugar phosphate chains. The helix has two
Flexibility of DNA
A model for the flexibility of DNA is proposed that is based on discrete variations in the direction of propagation in going from one subunit to the next, which gives a Gaussian distribution function.
A simple model of DNA superhelices in solution.
A very simple model of such a superhelix in which twisting and bending forces are in balance is described, subject both to topological constraints and to a limitation on the distance of closet approach of the interwound duplexes of thesuperhelix.
Cancer chemotherapy handbook
This book contains information on the biotechnology agents for cancer treatment and support and the use of new recombinant proteins.
Refinement of Large Structures by Simultaneous Minimization of Energy and R Factor
An improved method of crystallographic structure refinement, especially suitable for large molecules, is described, it is based on simultaneous minimization of a realistic potential-energy function
High-salt d(CpGpCpG), a left-handed Z′ DNA double helix
The DNA tetramer d(CpGpCpG) or CGCG crystallizes from high-salt solution as a left-handed double helix, the Z′ helix. Its structure differs from that of the other known left-handed helix, Z-DNA, by a