Crystal structure analysis of a complete turn of B-DNA

  title={Crystal structure analysis of a complete turn of B-DNA},
  author={R. Wing and H. Drew and T. Takano and C. Broka and Shoji Tanaka and K. Itakura and R. Dickerson},
DNA is probably the most discussed and least observed of all biological macromolecules. Although its role in biology is a central one, with many examples such as operators and restriction sites where specific base sequences have control functions or interact with specific enzymes, the structures that DNA can adopt have been based until now only on sequence-averaged fibre diffraction patterns. Recent improvements in triester synthesis methods have made possible the preparation of sufficient… Expand
The anatomy of A-, B-, and Z-DNA.
Single-crystal x-ray analyses of double-stranded DNA molecules of predetermined sequence, with 4 to 12 base pairs, are carried out for understanding of the mode of bending of DNA in chromatin, and the way in which specific DNA sequences are recognized by drug molecules and repressor proteins. Expand
Sequence dependence of the helical repeat of DNA in solution
The application of the band-shift method previously developed in this laboratory to the determination of the dependence of the DNA helical repeat in solution on its nucleotide sequence is reported. Expand
New crystal structures of nucleic acids and their complexes.
In the past year, X-ray crystallographic studies of representatives of all nucleic acid structural types have been reported and the structure that has received most attention is that of the first complex of a ribozyme with an inhibitor DNA. Expand
X-Ray Analysis of Oligonucleotides and the Changing Concept of DNA Structure
X-ray fibre diffraction studies played a crucial part in the discovery of the double helix and the role of DNA in the transfer of genetic information [1]. During the past thirty years the parametersExpand
One- and Two-Dimensional NMR Studies of the Structures of Simple Sequence DNAs
Progress towards understanding the biological functions of proteins and DNA at the molecular level is moving at a very rapid pace as a result of the development of DNA sequencing and footprintingExpand
Structural variations inZ-DNA
| The first crystal structure of Z-DNA, i.e. of the sequence d(CGCGCG)2 established many sequence-dependent micro-structural features. Nevertheless, a regular model of Z-DNA could be built forExpand
Crystallization and crystal packing analysis of DNA oligonucleotides
Abstract There are now over 30 DNA oligonucleotides that have been crystallized and their structure determined by X-ray diffraction analysis. From these studies there is a new wealth of informationExpand
DNA: Sequence, Structure and Supercoiling
Recent single-crystal studies have allowed for the first time atomic, or nearly atomic, resolution structures to be elucidated, giving information on each nucleotide in a molecule rather than an averaged structure, suggesting that DNA structure exhibits a large element of sequence dependence. Expand
Structural variations in Z-DNA
The first crystal structure of Z-DNA, i.e. of the sequence d(CGCGCG)2 established many sequence-dependent micro- structural features. Nevertheless, a regular model of Z-DNA could be built forExpand
Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H:DNA Complexes.
This work describes an alternative approach to determine crystal structures of the dodecamer in cases where molecular replacement does not produce a solution or when crystals of the DNA alone cannot be grown. Expand


How many base-pairs per turn does DNA have in solution and in chromatin? Some theoretical calculations.
  • M. Levitt
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1978
Calculations on a 20-base pair segment of DNA double helix using empirical energy functions show that DNA can be bent smoothly and uniformly into a superhelix with a small enough radius (45 A) to fitExpand
Helical repeat of DNA in solution.
  • J. Wang
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1979
The helical repeat of DNA in solution has been measured directly by analyzing the gel electrophoretic patterns of pairs of covalently closed DNAs with length differences between 1 and 58 base pairs,Expand
Sequence-specific recognition of double helical nucleic acids by proteins.
It is concluded that a single hydrogen bond is inadequate for uniquely identifying any particular base pair, as this leads to numerous degeneracies, but using two hydrogen bonds, fidelity of base pair recognition may be achieved. Expand
Molecular structure of a left-handed double helical DNA fragment at atomic resolution
The DNA fragment d(CpGpCpGpCpG) crystallises as a left-handed double helical molecule with Watson–Crick base pairs and an antiparallel organisation of the sugar phosphate chains. The helix has twoExpand
Flexibility of DNA
A model for the flexibility of DNA is proposed that is based on discrete variations in the direction of propagation in going from one subunit to the next, which gives a Gaussian distribution function. Expand
High-salt d(CpGpCpG), a left-handed Z′ DNA double helix
The DNA tetramer d(CpGpCpG) or CGCG crystallizes from high-salt solution as a left-handed double helix, the Z′ helix. Its structure differs from that of the other known left-handed helix, Z-DNA, by aExpand
A simple model of DNA superhelices in solution.
A very simple model of such a superhelix in which twisting and bending forces are in balance is described, subject both to topological constraints and to a limitation on the distance of closet approach of the interwound duplexes of thesuperhelix. Expand
Transient electric dichroism of rod-like DNA molecules.
The results establish transient electric dichroism measurements as a technique well suited for study of alterations in the length and base pair inclination of rod-like DNA molecules. Expand
Refinement of Large Structures by Simultaneous Minimization of Energy and R Factor
An improved method of crystallographic structure refinement, especially suitable for large molecules, is described, it is based on simultaneous minimization of a realistic potential-energy functionExpand
Cancer chemotherapy handbook
This book contains information on the biotechnology agents for cancer treatment and support and the use of new recombinant proteins. Expand