Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems.

@article{Yamada2017CrystalSO,
  title={Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems.},
  author={Mari Yamada and Yuto Watanabe and Jonathan S Gootenberg and Hisato Hirano and Fei Ann Ran and Takanori Nakane and Ryuichiro Ishitani and Feng Zhang and Hiroshi Nishimasu and Osamu Nureki},
  journal={Molecular cell},
  year={2017},
  volume={65 6},
  pages={1109-1121.e3}
}
The RNA-guided endonuclease Cas9 generates a double-strand break at DNA target sites complementary to the guide RNA and has been harnessed for the development of a variety of new technologies, such as genome editing. Here, we report the crystal structures of Campylobacter jejuni Cas9 (CjCas9), one of the smallest Cas9 orthologs, in complex with an sgRNA and its target DNA. The structures provided insights into a minimal Cas9 scaffold and revealed the remarkable mechanistic diversity of the… CONTINUE READING