Crystal Structure of an IHF-DNA Complex: A Protein-Induced DNA U-Turn

  title={Crystal Structure of an IHF-DNA Complex: A Protein-Induced DNA U-Turn},
  author={P. Rice and Shu-wei Yang and K. Mizuuchi and H. Nash},
  • P. Rice, Shu-wei Yang, +1 author H. Nash
  • Published 1996
  • Biology, Medicine
  • Cell
  • Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA and functions as an architectural factor in many cellular processes in prokaryotes. Here, we report the crystal structure of IHF complexed with 35 bp of DNA. The DNA is wrapped around the protein and bent by >160 degrees, thus reversing the direction of the helix axis within a very short distance. Much of the bending occurs at two large kinks where the base stacking is interrupted by intercalation of a… CONTINUE READING
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