Crystal Structure of an IHF-DNA Complex: A Protein-Induced DNA U-Turn

  title={Crystal Structure of an IHF-DNA Complex: A Protein-Induced DNA U-Turn},
  author={P. Rice and Shu-wei Yang and K. Mizuuchi and H. Nash},
  • P. Rice, Shu-wei Yang, +1 author H. Nash
  • Published 1996
  • Biology, Medicine
  • Cell
  • Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA and functions as an architectural factor in many cellular processes in prokaryotes. Here, we report the crystal structure of IHF complexed with 35 bp of DNA. The DNA is wrapped around the protein and bent by >160 degrees, thus reversing the direction of the helix axis within a very short distance. Much of the bending occurs at two large kinks where the base stacking is interrupted by intercalation of a… CONTINUE READING
    695 Citations

    Figures, Tables, and Topics from this paper

    A good turn for DNA: the structure of integration host factor bound to DNA.
    • 34
    • Highly Influenced
    Structure-based analysis of HU-DNA binding.
    • 84
    Extreme DNA Bending: Molecular Basis of the Regulatory Breadth of IHF
    • 8
    • Highly Influenced


    Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees
    • 973
    • PDF
    Single base-pair precision and structural rigidity in a small IHF-induced DNA loop.
    • 30
    The interaction of E. coli IHF protein with its specific binding sites
    • 230
    Co-crystal structure of TBP recognizing the minor groove of a TATA element
    • 928
    Stereochemical basis of DNA bending by transcription factors.
    • 95
    1.9 Å resolution refined structure of TBP recognizing the minor groove of TATAAAAG
    • 189
    • PDF
    The regulation of transcription initiation by integration host factor
    • 228