Crystal Structure of a Group I Ribozyme Domain: Principles of RNA Packing

@article{Cate1996CrystalSO,
  title={Crystal Structure of a Group I Ribozyme Domain: Principles of RNA Packing},
  author={Jamie H. D. Cate and Anne R Gooding and Elaine R. Podell and Kaihong Zhou and Barbara L Golden and Craig E Kundrot and Thomas R. Cech and Jennifer A. Doudna},
  journal={Science},
  year={1996},
  volume={273},
  pages={1678 - 1685}
}
Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleotide domain, a sharp bend allows stacked helices of the conserved core to pack alongside helices of an adjacent region. Two specific long-range interactions… 
Joining the Two Domains of a Group I Ribozyme to Form the Catalytic Core
TLDR
Site-directed mutagenesis and kinetic analysis of RNA splicing were used to identify a base triple in the conserved core of both a cyanobacterial and a eukaryotic (Tetrahymena) group I intron, revealing exceptionally dense packing of RNA.
Metal-binding sites in the major groove of a large ribozyme domain.
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The structure of the active site of an active ribozyme derived from the orf142-I2 intron from phage Twort bound to a four-nucleotide product RNA is solved and reveals three potential binding sites for catalytic metals.
Crystal structure of a group I intron splicing intermediate.
TLDR
Models of the pre-first and pre-second steps of intron splicing are proposed with full-sized tRNA exons, suggesting that the tRNA undergoes substantial angular motion relative to the intron between the two steps of splicing.
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TLDR
The structure of a 29-nucleotide RNA containing the sarcin/ricin loop (SRL) of rat 28 S rRNA has been determined and comparisons with other RNA crystal structures establish the cross-strand A stack and the GNRA tetraloop as defined and modular RNA structural elements.
Contributions to the study of the architecture and evolution of ribozymes
TLDR
The pk-turn, a new RNA motif related to k-turns that allow for the formation of a bend of 60° between stems P16 and P17 from the bacterial RNaseP, is identified.
A minor groove RNA triple helix within the catalytic core of a group I intron
TLDR
Using nucleotide analog interference suppression (NAIS), it is demonstrated that the P1 substrate helix and J8/7 single stranded segment form an extended minor groove triple helix within the catalytic core of the ribozyme.
Structure and thermodynamics of metal binding in the P5 helix of a group I intron ribozyme.
TLDR
Structural and thermodynamic results provide systematic new information about major groove metal ion binding in RNA and can be understood in terms of the different structures of the corresponding metal ion-RNA complexes.
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  • Chemistry, Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1998
TLDR
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TLDR
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