Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements

@article{Korostelev2014CrystalSO,
  title={Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements},
  author={Andrei A. Korostelev and Sergei Trakhanov and Martin Laurberg and Harry F. Noller},
  journal={Cell},
  year={2014},
  volume={126},
  pages={1065-1077}
}
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References

SHOWING 1-10 OF 83 REFERENCES

X-ray crystal structures of 70S ribosome functional complexes.

Structures of 70S ribosome complexes containing messenger RNA and transfer RNA (tRNA), or tRNA analogs, have been solved by x-ray crystallography at up to 7.8 angstrom resolution. Many details of the…

Crystal Structure of the Ribosome at 5.5 Å Resolution

The crystal structure of the complete Thermus thermophilus 70S ribosome containing bound messenger RNA and transfer RNAs (tRNAs) at 5.5 angstrom resolution is described, suggesting coupling of the 20 to 50 angstrom movements associated with tRNA translocation with intersubunit movement.

Structure of the 30S ribosomal subunit

The crystal structure of the 30S subunit from Thermus thermophilus, refined to 3 Å resolution, is reported, which will facilitate the interpretation in molecular terms of lower resolution structural data on several functional states of the ribosome from electron microscopy and crystallography.

Identification of molecular interactions between P-site tRNA and the ribosome essential for translocation

  • Jason S FeinbergS. Joseph
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 2001
This work has used a fragmented P-site-bound tRNAMet to identify two 2′-hydroxyl groups at positions 71 and 76 in the 3′-acceptor arm that are essential for translocation.

Crystal Structures of the Ribosome in Complex with Release Factors RF1 and RF2 Bound to a Cognate Stop Codon

Structures of the Bacterial Ribosome at 3.5 Å Resolution

Swiveling of the head of the small subunit observed in the present structures, coupled to the ratchet-like motion of the two subunits observed previously, suggests a mechanism for the final movements of messenger RNA and transfer RNAs during translocation.

Ribosomes and translation.

Biochemical and genetic approaches have identified specific functional interactions involving conserved nucleotides in 16S and 23S rRNA and promise to yield an unprecedented view of the mechanism of translation in the coming years.

Function of the ribosomal E-site: a mutagenesis study

A study of Escherichia coli ribosomes with the E-site binding destabilized by mutation C2394G of the 23S rRNA, which affects formation of the P/E hybrid site and leads to a loss of stimulation of the multiple turnover GTPase activity of EF-G by deacylated tRNA bound to the ribosome.

Selection of tRNA by the Ribosome Requires a Transition from an Open to a Closed Form

A ratchet-like inter-subunit reorganization of the ribosome during translocation

Three-dimensional cryo-electron microscopy maps of the Escherichia coli 70S ribosome in various functional states show that both EF-G binding and subsequent GTP hydrolysis lead to ratchet-like rotations of the small 30S sub unit relative to the large 50S subunit, indicating a two-step mechanism of translocation.
...