Crystal Structure and Biochemical Characterization of a Mycobacterium smegmatis AAA-Type Nucleoside Triphosphatase Phosphohydrolase (Msm0858).

@article{Unciuleac2016CrystalSA,
  title={Crystal Structure and Biochemical Characterization of a Mycobacterium smegmatis AAA-Type Nucleoside Triphosphatase Phosphohydrolase (Msm0858).},
  author={Mihaela-Carmen Unciuleac and Paul J. Smith and Stewart Shuman},
  journal={Journal of bacteriology},
  year={2016},
  volume={198 10},
  pages={
          1521-33
        }
}
UNLABELLED AAA proteins (ATPases associated with various cellular activities) use the energy of ATP hydrolysis to drive conformational changes in diverse macromolecular targets. Here, we report the biochemical characterization and 2.5-Å crystal structure of a Mycobacterium smegmatis AAA protein Msm0858, the ortholog of Mycobacterium tuberculosis Rv0435c. Msm0858 is a magnesium-dependent ATPase and is active with all nucleoside triphosphates (NTPs) and deoxynucleoside triphosphates (dNTPs) as… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 27 references