Crystal Structure and Biochemical Characterization of a Mycobacterium smegmatis AAA-Type Nucleoside Triphosphatase Phosphohydrolase (Msm0858).

@article{Unciuleac2016CrystalSA,
  title={Crystal Structure and Biochemical Characterization of a Mycobacterium smegmatis AAA-Type Nucleoside Triphosphatase Phosphohydrolase (Msm0858).},
  author={Mihaela-Carmen Unciuleac and P. Smith and S. Shuman},
  journal={Journal of bacteriology},
  year={2016},
  volume={198 10},
  pages={
          1521-33
        }
}
  • Mihaela-Carmen Unciuleac, P. Smith, S. Shuman
  • Published 2016
  • Medicine, Biology
  • Journal of bacteriology
  • UNLABELLED AAA proteins (ATPases associated with various cellular activities) use the energy of ATP hydrolysis to drive conformational changes in diverse macromolecular targets. Here, we report the biochemical characterization and 2.5-Å crystal structure of a Mycobacterium smegmatis AAA protein Msm0858, the ortholog of Mycobacterium tuberculosis Rv0435c. Msm0858 is a magnesium-dependent ATPase and is active with all nucleoside triphosphates (NTPs) and deoxynucleoside triphosphates (dNTPs) as… CONTINUE READING

    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 29 REFERENCES
    Genomic organization and in vivo characterization of proteolytic activity of FtsH of Mycobacterium smegmatis SN2.
    7
    The C-Terminus of ClpC1 of Mycobacterium tuberculosis Is Crucial for Its Oligomerization and Function
    5
    Complete structure of p97/valosin-containing protein reveals communication between nucleotide domains
    343
    Functional characterization of AAA family FtsH protease of Mycobacterium tuberculosis.
    17
    A novel ATP‐dependent conformation in p97 N–D1 fragment revealed by crystal structures of disease‐related mutants
    111
    Structural Basis of ATP Hydrolysis and Intersubunit Signaling in the AAA+ ATPase p97.
    42
    Characterization of a Mycobacterium tuberculosis proteasomal ATPase homologue.
    107