Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts.

@article{Brescia2009CryptosporidiumPM,
  title={Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts.},
  author={Cristin C Brescia and Shannon M. Griffin and Michael W. Ware and Eunice A. Varughese and Andrey I Egorov and Eric N. Villegas},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 21},
  pages={6856-63}
}
Cryptosporidium is an important waterborne protozoan parasite that can cause severe diarrhea and death in the immunocompromised. The current methods used to monitor for Cryptosporidium oocysts in water are the microscopy-based USEPA methods 1622 and 1623. These methods assess total levels of oocysts in source waters, but do not determine oocyst viability or genotype. Recently, propidium monoazide (PMA) has been used in conjunction with molecular diagnostic tools to identify species and assess… CONTINUE READING

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