Cryotrapped reaction intermediates of cytochrome p450 studied by radiolytic reduction with phosphorus-32.

@article{Denisov2001CryotrappedRI,
  title={Cryotrapped reaction intermediates of cytochrome p450 studied by radiolytic reduction with phosphorus-32.},
  author={Ilia G Denisov and Thomas M Makris and Stephen G Sligar},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 15},
  pages={11648-52}
}
Unstable reaction intermediates of the cytochrome P450 catalytic cycle have been prepared at cryogenic temperatures using radiolytic one-electron reduction of the oxy-P450 CYP101 complex. Since a rate-limiting step in the catalytic cycle of the enzyme is the reduction of the ferrous oxygenated heme protein, subsequent reaction intermediates do not normally accumulate. Using (60)Co gamma-irradiation, the primary reduced oxy-P450 species at 77 K has been identified as a superoxo- or hydroperoxo… CONTINUE READING