Cryotolerance of in vitro-produced porcine blastocysts is improved when using glucose instead of pyruvate and lactate during the first 2 days of embryo culture.

@article{CastilloMartn2013CryotoleranceOI,
  title={Cryotolerance of in vitro-produced porcine blastocysts is improved when using glucose instead of pyruvate and lactate during the first 2 days of embryo culture.},
  author={M{\'i}riam Castillo-Mart{\'i}n and Marc Yeste and Roser Morat{\'o} and Teresa Mogas and Sergi Roca Bonet},
  journal={Reproduction, fertility, and development},
  year={2013},
  volume={25 5},
  pages={
          737-45
        }
}
The objective of the present study was to determine the effects of replacing glucose with pyruvate and lactate during the first 48 h of in vitro culture (IVC) in NCSU-23 medium on embryo development, embryo quality and survival of porcine blastocysts after vitrification. To this end, in vitro-produced (IVP) porcine oocytes were cultured with either glucose for 6 days (IVC-Glu) or pyruvate-lactate from Day 0 to Day 2 and then with glucose until Day 6 (IVC-PyrLac). Blastocysts were vitrified on… CONTINUE READING
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