Cryosurvival of human spermatozoa frozen in eight different buffer systems.

  title={Cryosurvival of human spermatozoa frozen in eight different buffer systems.},
  author={L Weidel and Gail S. Prins},
  journal={Journal of andrology},
  volume={8 1},
The present study was conducted to ascertain optimal cryoconditions for human spermatozoa by comparing the relative cryoprotective efficiency of eight buffer systems and assessing various cryovials and thaw rates under two freeze rates. Spermatozoa that were cryopreserved in one of four zwitterion buffers (TES-Tris-citrate-egg yolk-glycerol; TES-Tris-citrate-I) maintained higher progressive motility at 0, 1, 2, and 4 hours post-thaw as compared to cells frozen in glycerol only, citrate-egg yolk… CONTINUE READING


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