Cryopreserved Morulae can be used to Efficiently Generate Germline-transmitting Chimeras by Blastocyst Injection

@article{ParkerThornburg2005CryopreservedMC,
  title={Cryopreserved Morulae can be used to Efficiently Generate Germline-transmitting Chimeras by Blastocyst Injection},
  author={Janice V. Parker-Thornburg and Jennifer L. Alana and Chad N. Smith and Michelle A. Detry and Marta L. Rojas and Kedryn K Baskin},
  journal={Transgenic Research},
  year={2005},
  volume={14},
  pages={685-690}
}
The production of chimeric mice is a complex process, requiring the careful coordination of tissue culture cell growth, production of a large number (30–75) of competent blastocysts and the availability of appropriately timed pseudo pregnant female mice. Failure at any of these steps can impinge upon the rapid production of chimeras. One potential improvement for the efficient generation of chimeric mice would be the utilization of cryopreserved embryos suitable for injection. C57Bl/6 morulae… CONTINUE READING