Cryopreservation of zebrafish (Danio rerio) oocytes using improved controlled slow cooling protocols.

@article{Guan2008CryopreservationOZ,
  title={Cryopreservation of zebrafish (Danio rerio) oocytes using improved controlled slow cooling protocols.},
  author={Mo Guan and David M. Rawson and Tiantian Zhang},
  journal={Cryobiology},
  year={2008},
  volume={56 3},
  pages={204-8}
}
Cryopreservation of gametes provides a promising method to preserve fish genetic material. Previously we reported some preliminary results on cryopreservation of zebrafish (Danio rerio) oocytes using controlled slow cooling and determined the optimum cryoprotective medium and cooling rate for stage III zebrafish oocytes. In the present study, the effects of two different cryopreservation media, cryoprotectant removal method, final sample freezing temperature before LN(2) plunge, warming rate… CONTINUE READING