Cryopreservation of yellow-poplar and sweetgum embryogenic cultures

@article{Vendrame2004CryopreservationOY,
  title={Cryopreservation of yellow-poplar and sweetgum embryogenic cultures},
  author={Wagner Vendrame and Christopher P. Holliday and Paul M. Montello and D. N. R. Smith and Scott A. Merkle},
  journal={New Forests},
  year={2004},
  volume={21},
  pages={283-292}
}
Cryopreservation has become anessential tool for operational application offorest tree embryogenic cultures, due to thelong evaluation periods needed for treesregenerated from these cultures. Fiveyellow-poplar (Liriodendron tulipifera)and seven sweetgum (Liquidambar spp.)embryogenic culture lines werestored in liquid nitrogen for 48 hours, afterwhich they were thawed and tested for regrowthand ability to produce somatic seedlings.Combinations of two sorbitol pretreatments andthree… CONTINUE READING
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