Cryopreservation of spermatozoa from freeze-tolerant and -intolerant anurans.

  title={Cryopreservation of spermatozoa from freeze-tolerant and -intolerant anurans.},
  author={S G Beesley and Jon P Costanzo and Richard E. Lee},
  volume={37 2},
Spermatozoa of the freeze-tolerant wood frog (Rana sylvatica) were used to develop a general protocol for the frozen storage of amphibian spermatozoa. Tolerance of spermatozoa to cryoprotective agents and freezing in suspension (-80 degrees C) was determined from rates of sperm lysis and dual-fluorochrome vital dye assays. We tested the efficacy of four cryoprotectants (Me2SO, methanol, glycerol, and ethylene glycol), two supplements (fetal bovine serum or glutathione), and combinations of… CONTINUE READING


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