Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application.

@article{Ding2011CryopreservationOS,
  title={Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application.},
  author={Fuhong Ding and Santosh P. Lall and Jun Li and Jilin Lei and Melissa Rommens and Joyce E. Milley},
  journal={Cryobiology},
  year={2011},
  volume={63 1},
  pages={56-60}
}
Development of Atlantic halibut (Hippoglossus hippoglossus) aquaculture will be enhanced with cryopreservation of halibut sperm by ensuring a reliable supply of sperm of desired quality and quantity. To assist in its commercial application, the cryopreservation of large volumes of halibut sperm was investigated. Three cryoprotectants were compared: dimethyl sulfoxide (DMSO), polyethylene glycol (PG) and glycerol (GLY) at two concentrations (10% or 15%). Two salt solutions, Hanks' balanced salt… CONTINUE READING