• DOI:10.1134/S1021443707010189
• Corpus ID: 1575695

Cryopreservation of seeds and protocorms of rare temperate orchids

@article{Nikishina2007CryopreservationOS,
  title={Cryopreservation of seeds and protocorms of rare temperate orchids},
  author={T. V. Nikishina and Elena V. Popova and M. G. Vakhrameeva and T. I. Varlygina and Galina L Kolomeitseva and A. V. Burov and E. A. Popovich and A. I. Shirokov and V. Yu. Shumilov and A. S. Popov},
  journal={Russian Journal of Plant Physiology},
  year={2007},
  volume={54},
  pages={121-127}
}

• T. V. Nikishina, E. Popova, +7 authors A. Popov
• Published 1 February 2007
• Biology
• Russian Journal of Plant Physiology

The efficiency of cryopreservation of seeds of five rare and endangered species of temperate orchids belonging to Platanthera and Dactylorhiza genera followed by their asymbiotic culture in vitro, as well as of in vitro cultured D. fuchsii protocorms (specific stage of orchid embryo development after release from the seed coat) was investigated. Germination rates of seeds after their exposure to liquid nitrogen were species-depended and could be either higher or lower than in the unfrozen control. There was no significant difference between growth rates of protocorms of the same species obtained from seeds collected in various Russia regions and cultured for 5 months. After vitrification, 9% of D. fuchsii protocorms with a larger diameter of 1200 μm survived cryopreservation; however, their growth was retarded for three months when compared to control protocorms.