Cryopreservation of seeds and protocorms of rare temperate orchids

  title={Cryopreservation of seeds and protocorms of rare temperate orchids},
  author={T. V. Nikishina and Elena V. Popova and M. G. Vakhrameeva and T. I. Varlygina and Galina L Kolomeitseva and A. V. Burov and E. A. Popovich and A. I. Shirokov and V. Yu. Shumilov and A. S. Popov},
  journal={Russian Journal of Plant Physiology},
The efficiency of cryopreservation of seeds of five rare and endangered species of temperate orchids belonging to Platanthera and Dactylorhiza genera followed by their asymbiotic culture in vitro, as well as of in vitro cultured D. fuchsii protocorms (specific stage of orchid embryo development after release from the seed coat) was investigated. Germination rates of seeds after their exposure to liquid nitrogen were species-depended and could be either higher or lower than in the unfrozen… 
Cryopreservation of Orchids - A Review.
  • S. Kaur
  • Environmental Science
    Recent patents on biotechnology
  • 2019
The orchid seeds and pollen are the most suitable propagules for cryopreservation of orchids due to their minute size and less space requirement and among the tissues, seeds are theMost reliable.
In vitro conservation of Dendrobium germplasm
This review highlights 16 years of literature related to the preservation of Dendrobium germplasm and comprises the most comprehensive assessment of thorough studies performed to date, which shows reliable and reproducible results.
Study of Dactylorhiza Seeds (D. baltica and D. maculata) from the Orchid Collection of the Cryobank at Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
It was shown that comprehensive study of seed specimens collected from different habitats at different times may be synchronized by applying a cryopreservation technique and in vitro method for plant material cultivation.
Cryobiotechnology of Korean Orchid Biodiversity: A Case Study Using Cymbidium kanran
High regrowth (86–90%) of rhizome sections after cryopreservation was achieved following the combination of ABA-and-sucrose preculture with desiccation over silica-gel to water content below 34%.
A cryopreservation protocol for ex situ conservation of terrestrial orchids using asymbiotic primary and secondary (adventitious) protocorms
The protocols developed here provide a useful template for advancing cryoconservation of other orchid taxa, particularly rare and threatened species.
Cryopreservation the seeds of a Taiwanese terrestrial orchid, Bletilla formosana (Hayata) Schltr. by vitrification
This study improves, an efficient protocol was established that maintained seed viability and enhanced the germination rates of seeds, compared with previously described cryopreservation methods, and the germinated seeds showed normal morphology of both vegetative and reproductive organs.
Cryoprotectant solutions in star orchid seeds and bamboo orchid conservation in liquid nitrogen
An alternative for the conservation of orchid species threatened with extinction is the seeds preservation for long periods by cryopreservation. This work aimed to evaluate the influence of cryogenic
Cryopreservation of Orchid Seeds, Protocorms and Pollen
An efficient protocol for cryopreservation of hybrid mature seeds, protocorms, and pollen of Dendrobium Sw.
Cryopreservation of Orchid Genetic Resources by Desiccation: A Case Study of Bletilla formosana
This article describes cryopreservation of orchid genetic resources by desiccation and a case study of Bletilla formosana to provide a more practical potential cryopReservation method for future research needs.


Effect of Cryoconservation on Seed Germination of Rare Tropical Orchids
The feasibility of the conservation of mature seeds of some tropical orchids by freezing in liquid nitrogen was demonstrated, which offers opportunities for creating a cryobank ofOrchids.
The leaf flavonoids of the orchidaceae
Wild Orchids of Britain
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