Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration

@article{Khoddamzadeh2011CryopreservationOP,
  title={Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration},
  author={Amir Ali Khoddamzadeh and Uma Rani Sinniah and Paul Kieran Lynch and Midhzar Abdul Kadir and Saleh Bin Kadzimin and Maziah Mahmood},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2011},
  volume={107},
  pages={471-481}
}
Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsulation-dehydration approach. Various stages in obtaining successful cryopreservation using this method were optimized. Encapsulated PLBs precultured in half-strength MS medium supplemented with 0.75 M sucrose for 3 days exhibited the highest viability in terms of 2,3,5-triphenyltetrazoliumchloride (TTC) reduction. The amount of sucrose in the PLBs after incubation in different concentrations of… CONTINUE READING
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