Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration

  title={Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration},
  author={Amir Ali Khoddamzadeh and Uma Rani Sinniah and Paul Kieran Lynch and Midhzar Abdul Kadir and Saleh Bin Kadzimin and Maziah Mahmood},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsulation-dehydration approach. Various stages in obtaining successful cryopreservation using this method were optimized. Encapsulated PLBs precultured in half-strength MS medium supplemented with 0.75 M sucrose for 3 days exhibited the highest viability in terms of 2,3,5-triphenyltetrazoliumchloride (TTC) reduction. The amount of sucrose in the PLBs after incubation in different concentrations of… CONTINUE READING
10 Citations
80 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 10 extracted citations


Publications referenced by this paper.
Showing 1-10 of 80 references

Optimizing cryo-conservation strategies for scarce Malaysian orthodox rainforest tree germplasm using Taguchi experimental design. Biodiversity Conserv

  • J Muthusamy, HJ Staines, EE Benson, M Mansor, B Krishnapillay
  • 2005
Highly Influential
8 Excerpts

Cryopreservation for long-term conservation of agrobiodiversity: progress and prospects. A scientific lecture delivered at University Kebangsann Malaysia for the INBIOSIS seminar series

  • F Engelmann
  • 2009
Highly Influential
3 Excerpts

An encapsulation– dehydration protocol for cryopreservation of the azalea cultivar ‘No rd licht’ (Rhododendron simsii Planch.)

  • H Verleysen, EV Bockstaele, P Debergh
  • Sci Hortic
  • 2005
Highly Influential
2 Excerpts

encoding an extracellular deoxyribonuclease ( DNase ) from Aeromonas hydrophila

  • JT Chen, WC Chang
  • 2004
Highly Influential
1 Excerpt

Effects of complex organic extracts on callus growth and PLB regeneration through embryogenesis in the Doritaenopsis orchid

  • MO Islam, Rahman ARMM, S Matsui, Prodhan AKMA
  • Jpn Agric Res
  • 2003
Highly Influential
1 Excerpt

Similar Papers

Loading similar papers…