Cryopreservation of pluripotent stem cell aggregates in defined protein-free formulation.

@article{Sart2013CryopreservationOP,
  title={Cryopreservation of pluripotent stem cell aggregates in defined protein-free formulation.},
  author={S{\'e}bastien Sart and Teng Ma and Yan Hua Li},
  journal={Biotechnology progress},
  year={2013},
  volume={29 1},
  pages={143-53}
}
Cultivation of undifferentiated pluripotent stem cells (PSCs) as aggregates has emerged as an efficient culture configuration, enabling rapid and controlled large scale expansion. Aggregate-based PSC cryopreservation facilitates the integrated process of cell expansion and cryopreservation, but its feasibility has not been demonstrated. The goals of current study are to assess the suitability of cryopreserving intact mouse embryonic stem cell (mESC) aggregates and investigate the effects of… CONTINUE READING
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