Cryopreservation of mouse testicular tissue: prospect for harvesting spermatogonial stem cells for fertility preservation.

@article{Gouk2011CryopreservationOM,
  title={Cryopreservation of mouse testicular tissue: prospect for harvesting spermatogonial stem cells for fertility preservation.},
  author={Sok Siam Gouk and Yu Feng Jason Loh and Srinivasan Dinesh Kumar and Paul F Watson and Lilia L Kuleshova},
  journal={Fertility and sterility},
  year={2011},
  volume={95 7},
  pages={2399-403}
}
OBJECTIVE To investigate the efficacy of vitrification, rapid freezing, and slow freezing in preserving testicular tissue for subsequent isolation of spermatogonial stem cells. DESIGN Experimental study. SETTING University-based laboratory. ANIMALS Immature mouse testicular tissue. INTERVENTION(S) The tunica of the testis was manipulated before cryopreservation. The tunica was either breached with a fine needle or completely removed, or the testis was sectioned longitudinally into… CONTINUE READING
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Fundamentals and current practice of vitrification

  • L. Kuleshova
  • Borini A, Coticchio G, editors. Preservation of…
  • 2009
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