Cryopreservation of mouse ovarian tissue following prolonged exposure to an Ischemic environment.

@article{Cleary2001CryopreservationOM,
  title={Cryopreservation of mouse ovarian tissue following prolonged exposure to an Ischemic environment.},
  author={M. Cleary and M. Snow and M. Paris and J. Shaw and S. Cox and G. Jenkin},
  journal={Cryobiology},
  year={2001},
  volume={42 2},
  pages={
          121-33
        }
}
In cases in which ovarian tissue is to be cryopreserved for tissue or gene banking it is important to maintain its integrity and viability. This study examined how delays between the death of an animal and the collection/cryopreservation of its ovarian tissue influenced follicle viability. Mouse ovaries were placed in PBS+antibiotic (in vitro) or left within the body (in situ) at room temperature for 0, 3, 6, 12, or 24 h following the death of the donor. These ovaries were cryopreserved at 1… Expand
Survival of primordial follicles following prolonged transportation of ovarian tissue prior to cryopreservation.
TLDR
Transport of roughly isolated ovarian cortex cooled on ice for a period of up to 4 h allows survival of primordial follicles following cryopreservation and transplantation to immunodeficient mice. Expand
Fertility of mice following receipt of ovaries slow cooled in dimethyl sulphoxide or ethylene glycol is largely independent of cryopreservation equilibration time and temperature.
TLDR
The results show that, for the equilibration conditions examined, DMSO and EG are equally efficient cryoprotective agents for mouse ovarian tissue. Expand
Ovarian tissue cryopreservation and transplantation : approaches and techniques
TLDR
Evaluating the impact of tissue size and the processing time interval before cryopreservation (warm ischemia time) on follicular survival showed thatCryopreserved pieces measuring 5 mm2 maintain more of their histological features compared to smaller Chapter 2, and handling larger ovarian cortical pieces facilitates cryop Reservation as well as autotransplantation procedures. Expand
Cryopreservation of caprine ovarian tissue using glycerol and ethylene glycol.
TLDR
Cryopreservation of caprine PF in ovarian tissue was demonstrated using cryoprotectants from 1.5 and 3M glycerol (GLY) and ethylene glycol (EG) and ultrastructural analysis of PF frozen in 1.3M GLY as well as 3M EG demonstrated that these follicles remained morphologically normal. Expand
Cryopreservation of oocytes from pre-antral follicles.
TLDR
Additional studies are necessary for a better understanding of the events during follicular cryopreservation and to establish a standard protocol for ovarian transplantation or follicle culture. Expand
Ovarian Tissue Cryopreservation: Limitations and Perspectives for Female Fertility Preservation
TLDR
The present review seeks to approach the advantages and challenges of the cryopreservation of ovarian tissue, indicating the difficulties and challenges that encompass this procedure, with purpose of pointing out solutions to overcome the damaged tissue integrity and contact between reproductive and somatic cells. Expand
Follicle development in cryopreserved bitch ovarian tissue grafted to immunodeficient mouse.
TLDR
The results suggest that canine ovarian tissue can be successfully preserved by the slow-freezing protocol because the tissue showed follicular growth after xenotransplantation. Expand
Experimental studies on ovarian cryopreservation and transplantation
TLDR
The study showed that human post menopausal ovaries could be effectively cryopreserved in DMSO and that the stroma secreted androgens during in vitro perfusion and the vascularity showed a well-preserved morphology in these human ovaries. Expand
Cryopreservation of ovarian tissue: an emerging technology for female germline preservation of endangered species and breeds.
TLDR
The objective of this review is to summarize the current status of the cryopreservation of ovarian tissue in domestic species and non-endangered wild mammals as model for threatened and endangered species and breeds, and to provide new insights into techniques that can be applied in the future. Expand
Assessment of tissue injury in cryopreserved ovarian tissue.
TLDR
A study in a porcine model is conducted to investigate how freezing and thawing affects ovarian tissue and whether warm ischemia time before the cryopreservation process has any added detrimental effects. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 45 REFERENCES
Cryopreservation of murine ovarian tissue.
TLDR
The histology and endocrine function of autotransplanted cryopreserved ovaries were similar to those of nonfrozen transplanted ovaries and of ovaries in sham-operated groups, and both groups demonstrated folliculogenesis. Expand
Low temperature storage and grafting of human ovarian tissue.
  • R. Gosden
  • Medicine, Biology
  • Molecular and cellular endocrinology
  • 2000
TLDR
Ovarian tissue banking should still be regarded as an experimental procedure, though recent results indicate that it has clinical potential, and cryopreservation provides an opportunity for long-term preservation of oocytes. Expand
Effect of cryoprotectants on the survival of follicles in frozen mouse ovaries.
TLDR
Dimethylsulfoxide and 1,2-propanediol are the most effective cryoprotectants for 10-day-old mouse ovaries and the majority of follicles are lost during graft establishment. Expand
Allogeneic and xenogeneic transplantation of cryopreserved ovarian tissue to athymic mice.
TLDR
In vivo function and long-term survival of cryopreserved ovarian tissue after allogeneic or xenogeneic transplant were confirmed by the examination of vaginal cytology, and offspring were derived from allografts. Expand
Live births after autologous transplant of cryopreserved mouse ovaries.
TLDR
Following autologous transplantation of mouse ovaries, before or after cryopreservation, offspring appeared normal, with high rates of fertility. Expand
Follicular development in cryopreserved marmoset ovarian tissue after transplantation.
TLDR
The results suggest that freezing and thawing do not substantially damage marmoset ovarian tissue, and the cryopreserved tissue retains its ability to support the development of large antral follicles. Expand
Transplantation of cryopreserved fetal ovarian tissue to adult recipients in mice.
TLDR
It is demonstrated that cryopreserved fetal ovarian tissue can be transplanted to adult recipients with subsequent restoration of fertility and that this process is dependent on the absence of the ovaries of the recipients. Expand
Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue.
TLDR
Primordial follicles can be isolated from fresh and cryopreserved human ovarian tissue with similar high efficiency and viability rates and Electron microscopy confirmed that the majority of the cells lacked ultrastructural signs of damage after isolation andCryopreservation. Expand
Fundamental cryobiology of mammalian oocytes and ovarian tissue.
TLDR
Some of the advances in this area are described and the relative merits and limitations of several currently available egg and ovarian tissue cryopreservation procedures are outlined. Expand
The fertility of mice with orthotopic ovarian grafts derived from frozen tissue.
TLDR
Tissue treated with orthotopic ovarian grafts of tissue that had been frozen and stored at —79° C showed a remarkable capacity for reorganization and function, but the number of oocytes surviving was small and the reproductive life of the females bearing the grafts was curtailed in each of the four strains of mice used. Expand
...
1
2
3
4
5
...