Cryopreservation of kangaroo spermatozoa using alternative approaches that reduce cytotoxic exposure to glycerol.

@article{McClean2008CryopreservationOK,
  title={Cryopreservation of kangaroo spermatozoa using alternative approaches that reduce cytotoxic exposure to glycerol.},
  author={Rhett V McClean and Yeng Peng Zee and William Vincent Holt and Stephen D Johnston},
  journal={Cryobiology},
  year={2008},
  volume={57 3},
  pages={
          304-7
        }
}
Alternative techniques for the cryopreservation of kangaroo spermatozoa that reduced or eliminated the need for glycerol were investigated including; (1) freezing spermatozoa with 20% glycerol in pre-packaged 0.25 mL Cassou straws to enable rapid dilution of the glycerol post-thaw, (2) investigating the efficacy of 20% (v/v) dimethyl sulphoxide (DMSO) and dimethylacetamide (DMA-10%, 15% and 20% v/v) as cryoprotectants and (3) vitrification of spermatozoa with or without cryoprotectant (20% v/v… CONTINUE READING
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