Cryopreservation of in vitro-grown shoot-tips of hop (Humulus lupulus L.) using encapsulation/dehydration

@article{Martnez1999CryopreservationOI,
  title={Cryopreservation of in vitro-grown shoot-tips of hop (Humulus lupulus L.) using encapsulation/dehydration},
  author={Dayag Mart{\'i}nez and Ricardo S{\'a}nchez Tam{\'e}s and Maria Angeles Revilla},
  journal={Plant Cell Reports},
  year={1999},
  volume={19},
  pages={59-63}
}
 A cryopreservation procedure using encapsulation/dehydration was established for shoot-tips obtained from in vitro-grown shoots of hop. After dissection, shoot-tips were encapsulated in medium with alginate and 0.5 M sucrose. Optimal conditions consisted of preculture for 2 days in solid medium with 0.75 M sucrose, or in increasing sucrose concentrations, desiccation for 4 h with silicagel in a flow cabinet (16% water content) followed by rapid freezing and slow thawing. Shoot recovery after… CONTINUE READING