Cryopreservation of human peripheral blood lymphoid cells reactive to allogeneic antigens, virus-transformed autologous cells and autologous leukemia cells in in vitro sensitization.

@article{Yamada1982CryopreservationOH,
  title={Cryopreservation of human peripheral blood lymphoid cells reactive to allogeneic antigens, virus-transformed autologous cells and autologous leukemia cells in in vitro sensitization.},
  author={Hajime Yamada and Yoshihisa Kodera and Kazunari Yamada},
  journal={Nagoya journal of medical science},
  year={1982},
  volume={44 3-4},
  pages={117-31}
}
We asked if cryopreservation of human peripheral blood lymphoid cells (PBL) could maintain their function to generate cytotoxic activity against various antigens in vitro. Cells to be frozen were suspended in RPMI 1640 medium supplemented with 10% dimetylsulfoxide and 10% fetal calf serum at a concentration of I X 10' cells per mi. One and a halfml of this… CONTINUE READING