Cryopreservation of human ovarian tissue: comparison of novel direct cover vitrification and conventional vitrification.

@article{Zhou2010CryopreservationOH,
  title={Cryopreservation of human ovarian tissue: comparison of novel direct cover vitrification and conventional vitrification.},
  author={Xin-hui Zhou and Yi-jun Wu and Jin Shi and Ya-xian Xia and Shu-sen Zheng},
  journal={Cryobiology},
  year={2010},
  volume={60 2},
  pages={101-5}
}
OBJECTIVE To compare the effect of novel direct cover vitrification (DCV) and conventional vitrification (CV) for human ovarian tissue. STUDY DESIGN Ovarian biopsy specimens obtained from 12 patients were randomly allocated into five groups: Fresh, DCV1, DCV2, DCV3 and CV. Three concentrations of cryoprotectants were used in DCV group. The equilibration solution of DCV1, DCV2, DCV3 was 5% EG+5% DMSO+DPBS, 7.5% EG+7.5%DMSO+DPBS, 10% EG+10% DMSO+DPBS, respectively. And the vitrification… CONTINUE READING
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