Cryopreservation of embryonic cerebral tissue of rat.

@article{Fang1992CryopreservationOE,
  title={Cryopreservation of embryonic cerebral tissue of rat.},
  author={Jinyun Fang and Zhu X Zhang},
  journal={Cryobiology},
  year={1992},
  volume={29 2},
  pages={267-73}
}
Embryonic cerebral tissues (ECT) either fresh or frozen-stored, were cultured and transplanted into the cerebella of neonatal host rats. Many variables including composition of the freezing medium, freezing and thawing rates, and storage time in liquid nitrogen were studied systematically. The results indicated that the following conditions yielded good results for tissue culture: using 1 M Me2SO as the cryoprotectant, freezing the brain tissues at a rate of 1 degrees C/min until it reached -70… CONTINUE READING

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