Cryopreservation of embryogenic cultures of Quercus robur using desiccation and vitrification procedures.

@article{Martnez2003CryopreservationOE,
  title={Cryopreservation of embryogenic cultures of Quercus robur using desiccation and vitrification procedures.},
  author={Mar{\'i}a Teresa Mart{\'i}nez and Antonio Mart{\'i}n Ballester and Ana Mª Vieitez},
  journal={Cryobiology},
  year={2003},
  volume={46 2},
  pages={
          182-9
        }
}
Oak embryogenic cultures are generally maintained by repetitive embryogenesis. To facilitate management of embryogenic lines and limit the risks of somaclonal variation and contamination a cryopreservation protocol should be developed. In this work we investigated the ability of several pre-treatments to enable 4-6mg clumps (1.0-1.5mm) of globular-heart stage somatic embryos of Quercus robur to withstand freezing in liquid nitrogen. In the best of the two embryogenic culture lines used, 56% of… CONTINUE READING
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