Cryopreservation of embryogenic callus of Dioscorea bulbifera by vitrification.

  title={Cryopreservation of embryogenic callus of Dioscorea bulbifera by vitrification.},
  author={Sen-rong Hong and M Yin and Xinjian Shao and Aoxue Wang and Weilin Xu},
  journal={Cryo letters},
  volume={30 1},
Cryopreservation of callus of Dioscorea bulbifera by vitrification was optimized. Calli of Dioscorea bulbifera were pretreated in liquid Murashige and Skoog (MS) medium supplemented with 2 mg L(-1) kinetin (KT), 0.5 mg L(-1) NAA, 0.5 mg L(-1) 2,4-D and 0.2 M sucrose for 5 d under continuous light (36 microM m(-2) s(-1)) at 25 + or - 1 degree C. The material was then loaded with 60 percent vitrification solution (PVS2) for 20 min at room temperature and dehydrated with 100 percent PVS2 for 30… CONTINUE READING
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