Cryopreservation of embryogenic calli of cassava using sucrose cryoprotection and air desiccation

@article{Danso2003CryopreservationOE,
  title={Cryopreservation of embryogenic calli of cassava using sucrose cryoprotection and air desiccation},
  author={K. E. Danso and Brian V. Ford-Lloyd},
  journal={Plant Cell Reports},
  year={2003},
  volume={22},
  pages={623-631}
}
A simplified technique which simultaneously induces and cryoprotects embryogenic calli using sucrose followed by dehydration was developed for the cryopreservation of cassava genetic resources. An initial experiment to optimise the sucrose concentration needed for both embryo production and cryoprotection showed that higher concentrations of sucrose—between 0.4 M and 0.5 M—significantly reduced the viability as well as the number of embryos produced by the embryogenic clumps in the absence of… CONTINUE READING

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