Cryopreservation of cocoa (Theobroma cacao L.) somatic embryos for long-term germplasm storage

@article{Fang2004CryopreservationOC,
  title={Cryopreservation of cocoa (Theobroma cacao L.) somatic embryos for long-term germplasm storage},
  author={Jong-Yi Fang and A. Wetten and P. Hadley},
  journal={Plant Science},
  year={2004},
  volume={166},
  pages={669-675}
}
Cryopreservation using encapsulation-dehydration was developed for the long-term conservation of cocoa (Theobroma cacao L.) germplasm. Survival of individually encapsulated somatic embryos after desiccation and cryopreservation was achieved through optimization of cryoprotectants (abscisic acid (ABA) and sugar), duration of osmotic and evaporative dehydration, and embryo development stage. Up to 63% of the genotype SPA4 early-cotyledonary somatic embryos survived cryopreservation following 7… Expand
Cryopreservation of embryos: an overview.
  • F. Engelmann
  • Biology, Medicine
  • Methods in molecular biology
  • 2011
Effect of liquid nitrogen storage time on the survival and regeneration of somatic embryos of cocoa.
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