Cryopreservation of apple in vitro axillary buds using droplet-vitrification.

  title={Cryopreservation of apple in vitro axillary buds using droplet-vitrification.},
  author={Emiliano Condello and Emilia Caboni and Edwige Andre and B. Piette and R Druart and Rony Swennen and Bart Panis},
  journal={Cryo letters},
  volume={32 2},
In vitro axillary buds of two apple cultivars, Pinova and Jonagold, were successfully cryopreserved by droplet-vitrification. In vitro axillary buds of cv. Pinova were subjected to PVS2 for 15, 30, 45, 60, 80 or 100 min, while Jonagold buds were treated with PVS2 for 15, 30, 45 or 60 min. In addition, the effect of age of in vitro mother-plants on recovery after cryopreservation was evaluated. Recovery was performed on medium with various combinations of BA, IBA and GA3. Regrowth percentages… CONTINUE READING

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