• Biology
  • Published 2011

Cryopreservation of Umbilical Cord and Placenta

  title={Cryopreservation of Umbilical Cord and Placenta},
  author={Lu Lian-mei},
Objective:To establish a cryopreservation method for umbilical cord and placental tissue so as to provide enough cell source for gene therapy and cell treatment.Method:Umbilical cord and placental tissue were isolated from the body and cleaned up. DMSO was used as cryoprotectant and divided into four density grades:20kg/m~3,15kg/m~3,10kg/m~3,5kg/m~3.The temperature was declined gradually to -80℃,and then the tissue is putted into liquid nitrogen for cryopreservation.Fresh and resuscitated… CONTINUE READING