Cryopreservation of Grapevine (Vitis spp.) Embryogenic Cell Suspensions by Encapsulation–Vitrification

@article{Wang2004CryopreservationOG,
  title={Cryopreservation of Grapevine (Vitis spp.) Embryogenic Cell Suspensions by Encapsulation–Vitrification},
  author={Qiaochun Wang and Munir Mawassi and Nachman Sahar and Ping Li and Colova-Tsolova Violeta and Ron Gafny and Ilan Sela and Edna Tanne and Avihai Perl},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={77},
  pages={267-275}
}
Embryogenic cell suspensions of two grapevine rootstocks: 110 Ritcher (V. berlandieri × V. rupestris), 41B (V. vinifera × V. berlandieri) and several table grape and wine cultivars (Vitis vinifera) were successfully cryopreserved by the encapsulation–vitrification method. Embryogenic cell suspensions were precultured for 3 days in liquid MGN medium supplemented with daily increasing sucrose concentrations of 0.25, 0.5, 0.75 M. Precultured cells were encapsulated and directly dehydrated with a… CONTINUE READING
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