Cryopreservation of Ginkgo biloba cell culture: effect of pretreatment with sucrose and ABA.

@article{Lu2009CryopreservationOG,
  title={Cryopreservation of Ginkgo biloba cell culture: effect of pretreatment with sucrose and ABA.},
  author={Zhi-Wei Lu and Elena V. Popova and Chun-hua Wu and Eun-jung Lee and Eun Joo Hahn and K. N. Paek},
  journal={Cryo letters},
  year={2009},
  volume={30 3},
  pages={232-43}
}
The report describes the impact of preculture with sucrose and sucrose + ABA on desiccation and cryopreservation tolerance of cell cultures of Ginkgo biloba L., an important landscape and medicinal tree. Callus clumps were incubated on MS medium supplemented with high sucrose concentrations (up to 24 percent, w/v), employed alone or with ABA (2-10 mg per L) for various durations followed by desiccation for 0-240 min and cryopreservation. The beneficial effect of preculture on regrowth after… CONTINUE READING