Cryopreservation-induced alterations in protein composition of rainbow trout semen.

@article{Nynca2015CryopreservationinducedAI,
  title={Cryopreservation-induced alterations in protein composition of rainbow trout semen.},
  author={Joanna Nynca and Georg J. Arnold and Thomas Froehlich and Andrzej Ciereszko},
  journal={Proteomics},
  year={2015},
  volume={15 15},
  pages={
          2643-54
        }
}
The aim of this study was to detect cryopreservation-induced alterations in the protein composition of rainbow trout semen using two independent methods 1DE SDS-PAGE prefractionation combined with LC-MS/MS and 2D difference gel electrophoresis followed by MALDI-TOF/TOF identification. Here, we show the first comprehensive dataset of changes in rainbow trout semen proteome after cryopreservation, with a total of 73 identified proteins released from sperm to extracellular fluid, including… CONTINUE READING
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